Description
This Human GDNF Family Receptor Alpha-3 (GFRA3) ELISA Kit is intended for quantitative detection of human GFRA3 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Strip well format. Reagents for up to 96 tests.
This human GFRA3 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GFRA3 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: D32-N374) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GFRA3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GFRA3 amount of sample captured in plate.
The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: GDNF family receptor alpha-3 (GFRalpha3), also known as the artemin receptor, is a protein that in humans is encoded by the GFRA3 gene. It is mapped to 5q31.2. The protein encoded by this gene is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin